ChemBioChem, 2009, 10(10), 1605-1611
Analysis of Protein-Protein Interactions by Using Droplet-Based Microfluidics
Monpichar Srisa-Art*, Dong-Ku Kang*, Jongin Hong*, Hyun Park, Robin J. Leatherbarrow, Joshua B. Edel, Soo-Ik Chang, Andrew J. deMello
*equal contribution
Every little drop: The KD values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL-sized droplets agree with data from bulk-fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodies to ANG. Such an experimental platform could be applied to the high-throughput analysis of protein-protein interactions.
http://www3.interscience.wiley.com/journal/122428316/abstract
